Kinetics of CD4‐1+ lymphocytes in brown trout after exposure to viral haemorrhagic septicaemia virus

Abstract T‐helper cells express CD4 as a co‐receptor that binds to major histocompatibility complex class II synchronize the immune response against upcoming threats via mediating several cytokines. We have previously reported presence of homologues in brown trout. The study cellular responses trout is limited by availability specific antibodies. here describe generation polyclonal antibody CD4‐1 allows for investigation + cells. used this novel tool different tissues during viral haemorrhagic septicaemia infection (VHSV) using flow cytometric technique. Flow analyses revealed an enhanced level surface expression infected group lymphoid organs and intestine. These results suggest important role within viruses, comparable higher vertebrates.